FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6589813
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG7953.2436856E-614.671528145
GGATCGG28700.013.817818145
GATCGGC19955.456968E-1211.693097145
CCGATCG7300.00549377811.184527145
GATCGGG20057.7852746E-1010.4713145
CACACGG51450.010.201631145
GATCGGT10456.6790683E-410.045433145
CACGTCG24252.0918378E-109.619682145
ATCGGAC10150.00592072969.193194145
ATATCGG11600.0017358289.04955145
TTGATCG11850.0021072578.858631145
CGATCGG7301.1523352E-67.988948145
AGGATCG25654.632875E-77.730437145
ATCGGAA46900.07.6023884135-139
AGGGGGG76350.07.24946451
GAGCACC93100.07.0158587145
GATCGGA44150.06.860962135-139
GGGATCG25903.408261E-56.7551336145
CGGGTCG20350.00547825826.3047915145
TGGGGGG152700.06.041221
Produced by FastQC (version 0.11.5)