FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11774860
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157240.133538742711166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT55500.020.671944
TATACTG93050.020.5747055
TACACTG92200.017.863485
TCTATAC55850.017.6492463
TAGACTG61100.017.16435
CTACACT66800.016.9643044
GTATAGG40150.016.859981
CCTATAC35600.016.6130983
TATACAG88350.016.2519235
GTGTAGG70200.016.1718671
GTATTAG53300.016.1400181
ATACTGT123300.016.097966
TACACAG125750.014.8326585
GTGCTAG58700.014.7753711
TCTACAC72250.014.7150063
GTATAGA65200.014.7083071
CCTACAC66550.014.7057793
TAGACAG99350.014.66505055
GTCCTAG53250.014.5661391
GTGTATA89200.014.4663061
Produced by FastQC (version 0.11.5)