FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13173430
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG94450.019.216265
CTATACT69000.018.3822614
TACACTG107550.017.462065
CCTATAC42100.016.3122333
GTATTAG67700.016.2516921
CTACACT80000.015.7671054
GTATAGG43850.015.5020711
TATACAG103700.015.4748995
TAGACTG75700.015.4593675
ATACTGT125800.015.2630396
CCTACAC73900.014.9824253
GTGTAGG75100.014.9302681
GTGTATA105100.014.6692381
GTCCTAG64900.014.5772861
CTAATAC67000.014.538163
CTAGGAC60850.014.5104023
TACACAG138800.014.4898395
TCTATAC66150.014.4071653
GTATAAG66650.014.4048261
TCTACAC82550.014.3462733
Produced by FastQC (version 0.11.5)