FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19197339
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244440.1273301471625833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG145300.017.1245385
TATACTG138150.017.1027185
GTGTAGG97300.016.4341751
GTATAGA109500.016.388691
CTACACT104600.016.2614964
GTGTATA149550.016.0619131
CCTACAC95650.015.9663783
TATACAG148200.015.2845085
GTATTAG91700.015.2295091
GTGTACG28550.015.1638971
CTAGGAC81400.014.9920463
TCTATAC99750.014.8906593
TACACAG194300.014.8147245
GTCTAGG83750.014.6741561
GTACTAG51350.014.5501871
GTATAGG61300.014.4666681
CTTACAC108550.014.3901553
CCTATAC64000.014.3827283
CTAGACT81850.014.3084614
CTATACT99500.014.292544
Produced by FastQC (version 0.11.5)