FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16569661
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG133400.018.8711765
CTACACT91800.018.7668424
TATACTG131400.018.4091955
TCTATAC80950.016.9388643
GTGTAGG95100.016.566411
CTATACT82050.016.4547184
GTATACG15500.016.3354721
GTATAGG53000.016.0572221
TATACAG118550.016.0152595
GTATAAG76350.015.7524441
TAGACTG88050.015.4134445
GTATATA198850.015.350591
GTGTATA120500.015.23401451
GTCCTAG79150.015.1951881
CTATACA92850.015.0708934
ATACTGT166850.015.0459286
TACACAG168300.014.9161215
CCTACAC90100.014.8284353
TACACCG27650.014.7504275
GTGTAGA101850.014.6398191
Produced by FastQC (version 0.11.5)