FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16569661
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214650.12954399006714742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG124250.020.9503175
TACACTG137600.018.5086785
CTATACT81750.017.5560864
CTACACT95100.016.9410464
GTATAGG52550.015.9466261
ATACTGT162750.015.8646916
TAGACTG89850.015.7384975
GTATTAG75600.015.5556871
GTGTAGG95750.015.4445431
CCTGTCG60750.015.371616145
CTAGACT74550.015.2880694
GTGTATA124850.015.2853481
TACACAG170050.015.0594825
GTATATA201600.014.7755771
TTATACT135600.014.7347884
TCTATAC82250.014.7122513
CCTACAC87200.014.441863
GTGTTAG97350.014.1779911
CTAATAC85600.014.1364793
GTCCTAG77250.014.0383741
Produced by FastQC (version 0.11.5)