FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15275786
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185090.12116561465315107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG40700.022.383709145
TATACTG121050.018.117345
TACACTG127100.016.8751225
GTGTAGG91100.015.911041
CTACACT93400.015.8015244
CACATCG59400.015.531126145
GTCCTAG72900.015.4332631
TCTATAC84550.015.1718013
GTATTAG84450.015.1206251
CTAGGAC69200.014.7500463
GTATAGG52550.014.5796791
GTACTAG44550.014.408971
TGTCTCG99250.014.291374145
CCTACAC78500.014.2325643
GTCTTAG77300.014.1083181
ATACTGT167200.014.0653386
GTGTATA131650.013.9987131
ACACTGT180500.013.7548986
TAGACTG88700.013.6842545
GTGCTAG76850.013.5622181
Produced by FastQC (version 0.11.5)