FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16140262
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG101800.013.7169465
CTATACT71300.012.9238374
GTATTAG64100.012.4977571
CCTATAC47250.012.1512473
GTATAGG51500.011.9763281
GTGTATA97400.011.7186771
GTATAGA67000.011.4277471
GTATATA146750.011.3527591
GTGTTAG82650.011.322511
TACACTG124700.011.2547985
CTAGACT60400.011.1487024
TCTATAC66150.010.9296933
ATACTGT136350.010.4491246
TATACAG97400.010.3339985
GTATATG95850.010.2809761
GTGTAGA96400.010.2223191
CCTAGAC64300.010.1417873
TAGACTG81800.010.0517965
CTAGGAC66100.09.9728463
GTCTTAG66100.09.8672931
Produced by FastQC (version 0.11.5)