FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16140262
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169910.1052709057634876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG97600.013.3676785
GTGTATA98050.012.1580721
TATACAG101900.011.8293995
CTATACT71850.011.6445574
TACACTG117700.011.5667865
GTGTTAG81500.011.3184991
GTATAAG66250.011.2462181
CTAGACT59850.011.1360574
GTATAGA70750.011.0323821
GTATATA144900.010.479691
CCTATAC47300.010.3431233
GTATAGC45600.010.2702771
GCTATAC42450.010.1886313
GTCTAGG71000.010.0940661
TCTAGAC62950.09.9117573
GTGTAGG104150.09.8789731
GTCTAGA65750.09.8208421
TACACAG158600.09.7910415
GTATATG100750.09.7897771
TATATAC138150.09.7513893
Produced by FastQC (version 0.11.5)