FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12737924
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG42400.021.0880681
TATACTG93100.019.4996415
TACACTG101450.018.5882875
CTATACT64050.017.6870274
TCTATAC57700.016.7067173
TAGACTG69750.016.2419535
GTGTAGG76500.016.1055491
CTACACT72000.015.929544
TACACAG135100.015.7813415
ATACTGT119800.015.5061416
GTGTTAG71300.015.502761
GTATTAG56050.015.3243871
GTGTAGA80400.015.2367411
GTCCTAG61250.015.1728351
GTGTATA95200.015.160581
TAGGACT67300.014.950964
CCTACAC72600.014.9255553
TATACAG93750.014.8610865
GTGTAAG76350.014.661791
GTATATA144150.014.5546061
Produced by FastQC (version 0.11.5)