FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12737924
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165430.1298720262422668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG103750.019.9310155
TATACTG88250.019.765475
CTACACT74100.017.8452844
TATACAG88950.016.6051775
GTGTAGG74700.016.3178161
CTATACT56250.016.0055754
CTTACAC70800.015.2049593
GTATAGG41000.015.1229091
CCTATAC39550.015.1216623
CTAGGAC58650.015.1157483
CTAGACT54500.015.099874
TCTATAC61200.015.0607633
GTGCTAG59950.014.9262621
GTATTAG57600.014.9236081
GTGTAAG75900.014.5745241
TACACAG137800.014.5467465
ATACTGT120000.014.4777566
GTGTATA95800.014.3418651
GTGTTAG71850.014.3173491
GTATTAC57700.014.1650681
Produced by FastQC (version 0.11.5)