FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8679691
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG65750.020.0953525
CCTACAC46200.019.6236023
CTACACT50850.018.1055344
TACACTG69550.016.8753535
GTGTAGG52150.016.850271
GTATTAG43950.016.4751551
CTATACT45800.016.4190984
GTATAGA48500.016.2340721
GTATAGG30800.015.7488761
CCTATAC28750.015.400463
GTATAAG41650.015.0219451
GTCTTAG40050.014.7444291
TAGACAG77800.014.7245325
ATACTGT90900.014.6900496
CTAGGAC42650.014.500893
GTATATA117400.014.1916021
GTGTATA72600.014.04051
TCTATAC45850.013.9486793
GTCTAGG41150.013.8377771
TATACAG65500.013.7341635
Produced by FastQC (version 0.11.5)