FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8679691
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG64400.019.1125645
CTACACT50100.018.9519024
TACACTG66950.018.3846045
TCTATAC43100.017.1336063
CTATACT45550.016.676014
CTAGGAC38000.016.6569583
TATACAG64900.016.5811085
CCTATAC28600.016.4757613
GTATAGG29250.015.8794441
GTATTAG43350.015.2600531
GTGTATA73350.014.8712971
GTATAGA47700.014.753631
ATACTGT88700.014.749126
TCTAGAC41200.014.6804043
GTATATA116700.014.5935771
GTGTACG14050.014.5257621
CCTAGAC38950.014.4450593
GTCCTAG40750.014.3340111
TAGACAG75350.014.2815375
TAATACT75200.014.21616654
Produced by FastQC (version 0.11.5)