FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13107086
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157980.12053022311748011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT57150.017.4467494
TATACTG83150.017.1660065
TACACTG100050.016.8069925
GTATAGG40500.016.2625181
CTACACT73250.016.014164
CCTATAC40500.015.7685013
CTTACAC71300.015.1718293
TCTATAC57300.015.0120053
GTCTAGG57800.015.005321
CCTACAC72300.014.9619843
GTGTAGG69600.014.7099721
TATACAG87700.014.5661195
GTATAGA61150.014.50320151
GTGTACG22200.014.3934371
GTGTATA83350.014.0827781
GTCCTAG52900.013.9297781
CTAGGAC55300.013.90516953
GTATATA123800.013.4846831
GTATTAG53750.013.2242011
GTGTTAG62150.013.2205911
Produced by FastQC (version 0.11.5)