FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16177589
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG112750.021.235515
GTGTAGG92300.017.7315881
ATACTGT155300.017.3893666
CTATACT74450.016.7342594
TACACTG128100.016.6262045
TATACAG112650.016.3114875
GTATAGA80800.016.2042141
GTGTATA111300.016.0812361
GTATATA175750.015.7713611
GTGTAGA99050.015.5388641
TCTATAC73800.015.4668883
GTATAGG51350.015.3256691
CCTATAC52800.015.0274873
TAGACTG86650.014.8601775
CTAGACT71700.014.7551154
CTACACT91850.014.5492354
GTATAAG74550.014.386481
CCTAGAC75350.014.3173913
TACACAG167600.014.2858235
CTAGGAC70850.014.2443823
Produced by FastQC (version 0.11.5)