FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14513249
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164590.11340672236795496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG48250.025.332558145
CCTGTCG75800.017.402525145
TGTCTCG101900.017.101858145
TACACTG118100.016.5175725
TATACTG104750.016.3516185
TAGATCG18850.015.408306145
TAGACTG80250.015.3301985
CTAGGAC60300.015.3299353
CACATCG62950.015.187502145
CTATACT73300.014.8364594
GTGTAAG82650.014.4858791
GTATTAG70250.014.4282941
CCTATAC47400.014.0529173
TATACCG14650.013.9186745
GTCCTAG66250.013.8619061
GTATAGG50200.013.822021
CTAGCAC67900.013.8142733
CTAGACT66700.013.7569494
GTGTAGG85200.013.6531091
TCTATAC73700.013.5571553
Produced by FastQC (version 0.11.5)