FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448239
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCG19200.017.71979145
TACACTG101300.016.7732355
GTGTATA105650.016.7523981
ACTGATC84700.016.5068958
TATACTG92450.016.4124265
GTGTAGG76900.015.9198021
CCTATAC43650.015.8590143
CTATACT64800.015.6464734
GTATTAG60800.015.5330281
CCTGTCG56100.015.475145
TGTCTCG64900.015.365122145
CTACACT73000.015.2299244
GTCCTAG61250.015.0762661
CTAGGAC59000.014.8143713
ATACTGT118550.014.7454626
TATACAG98950.014.4863195
TACACAG144100.014.314565
TAGACTG76050.014.0673475
CTAGACT57950.013.8761134
CCTAGAC62800.013.4725863
Produced by FastQC (version 0.11.5)