FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448239
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134510.10805544462955766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG24800.019.671556145
TACACTG101950.018.7352055
TATACTG94300.018.1034415
ACTGATC85750.015.9134958
CCTACAC73250.015.57340053
CTACACT78550.015.4098584
GTATTAG64000.015.3274011
CTAGGAC54350.015.3232493
CTATACT69900.014.7142754
TGTCTCG62800.014.611963145
TAGATCG14553.6379788E-1214.369796145
GTACTAG33650.014.3676091
GTGTATA110900.014.2789871
TGCACTG163250.014.2287885
GTATAGA73050.014.0999511
GTATATA168750.013.8682981
CCTGTCG55300.013.863086145
GTGTATG106500.013.5530831
TCTATAC63550.013.4353123
ACAGTGC129200.013.4324198
Produced by FastQC (version 0.11.5)