FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15219419
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG225170.14794914313089086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG105400.018.9132925
CTATACT71850.017.9124644
TCTATAC68200.016.4108053
TACACTG118400.016.1868295
GTGTAGG81750.016.021571
CTAGGAC67550.015.4296193
TATACAG108850.015.2936595
CTTACAC86200.014.9315473
TACACAG149000.014.5525245
GTATTAG68850.014.5473311
CCTATAC46000.014.4464143
ATACTGT148100.014.4048626
TAGACTG79150.014.4045465
GTATAAG67650.014.391241
GTGCTAG72350.014.3276321
GTGTATA103850.014.2981741
TAGGACT76800.014.20782854
CCTACAC81900.014.1781143
GTATATA156000.014.1428581
CTAGACT63500.014.0993994
Produced by FastQC (version 0.11.5)