FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10573092
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67700.019.5895735
CTATACT47050.018.888334
CCTATAC31600.018.820973
TACACTG81450.017.121865
GTATAGA51500.016.9971851
GTATTAG44150.016.4191041
GTGTAGG61750.016.3907571
CTACACT58100.016.11964
GTCTAGG48600.016.041421
TCTATAC46150.015.997863
CCTACAC61850.015.6948983
TACACAG106650.015.6401485
TATACAG71450.015.1170415
TAGACAG86100.015.0855925
GTATACG9600.014.9596811
GTGTAAG57800.014.9079181
ATACTGT91150.014.8499346
TAGACTG52700.014.658175
GTGTAGC48400.014.4121591
CTATACA53250.014.37832454
Produced by FastQC (version 0.11.5)