FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10573092
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133860.12660440295043304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67950.018.717335
CCTACAC61100.018.4661143
TACACTG83650.018.2288535
TAGACTG51550.017.5091765
CCTATAC32600.017.4097793
CTACACT61950.017.2189964
TATACAG74350.017.1981245
CTATACT49750.016.905834
GTCTAGG48600.016.9016111
CTAGGAC48700.016.8494243
GTATTAG42500.016.4283681
GTCCTAG48600.016.3382241
CCTAGAC48000.015.9554673
GTATAGG33750.015.6170891
TACACAG108300.015.5319895
GTGTTAG55800.015.4567981
ACACTGT123700.015.0362016
GTGTAGG65150.014.814531
TAGGACT51100.014.719614
CTATACA54900.014.6971864
Produced by FastQC (version 0.11.5)