FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204818
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG104550.018.9226995
TACACTG111200.018.0399075
GTGTAGA88350.016.598811
GTCCTAG63900.015.6969351
ATACTGT127400.015.6368516
CTAGGAC62200.015.5696453
CTATACT66350.015.5341124
TAGACTG73250.015.3929225
CCTACAC81650.015.2495623
CTTACAC80400.015.1425043
CTACACT87400.014.7211784
TACACAG152250.014.720715
GTATAGG45950.014.4521811
CTAGACT59900.014.4352484
TAGGACA78300.014.2234594
ATAGGAC50350.014.1507293
CCTATAC47450.014.1408853
TAGACAG111300.014.1082055
TGCACTG170850.014.0088415
TGTACTG101600.013.9572945
Produced by FastQC (version 0.11.5)