FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204818
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG106950.017.6456725
TATACTG100700.016.8187185
CTACACT77300.016.7230384
CCTATAC46800.016.6913173
GTCCTAG64450.015.2465931
CTTACAC79050.014.9538223
GTATAGG48700.014.9199631
GTGTAGG91950.014.6001531
CCTAGAC66600.014.4277483
CTACCCT82500.014.3283184
CTATACT71750.014.2591244
CTAGGAC60800.014.21233
GTGTATA103500.014.1074821
TATACAG103250.013.9930555
TAGACTG74700.013.9737415
GTGTACA99850.013.8608331
ATACTGT127750.013.7989326
TACCCTG91850.013.6224665
ACAGTGC143150.013.5702488
GTATTAC59700.013.561851
Produced by FastQC (version 0.11.5)