FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15914255
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG129000.016.3586185
TAGACTG86900.015.976185
CTACACT99500.015.9064974
TATACTG123250.015.6574355
GTGTAGA102100.015.3656981
GTATAGG55200.014.8392691
GTGTATA114950.014.7350111
CCTACAC98700.014.6991393
GTCCTAG77300.014.4582581
TATACAG123300.014.2999125
CCTAGAC79800.014.0050853
TGCACTG212500.013.9486355
TCTATAC82950.013.8079853
CTAGACT72500.013.7875544
GTGTAGG107450.013.7607721
CTAGCAC82700.013.5979143
GTGTAAG88100.013.473791
GTATTAG67500.013.3693161
CTATACT82800.013.3299824
CTAGGAC80450.013.2879333
Produced by FastQC (version 0.11.5)