FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15914255
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG119050.017.1398325
TACACTG130250.017.104725
CCTACAC101500.016.137493
CTACACT101650.015.6354324
CCTATAC52450.015.4821183
CTATACT84050.014.7807724
TAGACTG92350.014.5798645
ACAGTGC174050.013.9573998
GTGTAAG90900.013.9105021
GTATAGG58150.013.8593741
TAGGACT81200.013.8465254
ACTGTGC175950.013.806688
ACACTGT174050.013.7575326
CTAGGAC78600.013.6867133
TCTAGAC78900.013.6346713
TGCACTG211300.013.5984535
CTTACAC90700.013.391243
GTATAAG76900.013.3712091
AGGACTG184150.013.2665985
GCTATAC51850.013.11799053
Produced by FastQC (version 0.11.5)