FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757534
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144500.11326640399312281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG87150.019.0684785
TACACTG99600.018.6561345
CTATACT59500.017.0882024
CCTATAC40550.016.9516373
GTATAGG41750.016.3188021
GTATACG11800.016.0714381
GTGTATA90950.016.0632571
GTATTAG52900.015.9330371
CTACACT73000.015.753114
GTACTAG30750.015.5323361
GTATAGA63900.015.3886371
TAGACTG68400.015.3769525
GTCTAGG57200.015.2264481
TCTATAC57850.015.1559483
GTCTAGA58500.015.1282141
GTCCTAG55450.015.0736491
CTAGGAC56900.015.0391753
TATACAG88300.014.9290525
GTGTAAG73850.014.8370321
GTATATA135400.014.8361211
Produced by FastQC (version 0.11.5)