FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15741694
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG105400.019.3811265
CTATACT74900.017.7788454
GTGTATA104200.016.3995741
GTATAGA77250.016.342341
GTATTAG66350.015.9785321
CTAGGAC67550.015.8945183
TACACTG122100.015.81670955
CCTATAC49800.015.6798073
GTCCTAG66700.015.3718351
TACACAG166100.014.9008395
GTATAGG53650.014.8207211
CCTAGAC66950.014.787333
ATACTGT143150.014.7571766
GTGTAGG92450.014.7116681
GTATAAG73250.014.6638261
GTATATA165250.014.5616921
CTAGACT66250.014.5226284
TAGACTG81450.014.3803755
CTACACT82400.014.2991924
TAGACAG125500.014.2215925
Produced by FastQC (version 0.11.5)