FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15741694
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107100.018.3502585
GTGTAGG89300.017.1065221
CCTATAC52900.016.33593
TACACTG120100.016.1318575
TAGACTG78250.015.7641835
GTATAGG51150.015.6827151
GTATATA165850.015.6457541
CTATACT75850.015.6196664
GTATAAG75300.015.4700071
GTATAGA84150.015.4179941
CTTACAC91000.015.2401313
TCTATAC70500.015.2232933
GTATTAG66100.014.8794451
GTCCTAG68250.014.8195291
GTGTATA107900.014.2223141
TATACAG114800.014.0840865
CTACACT88850.014.0402234
CTAGACT65000.013.9382554
GTATACG16550.013.908651
GTACTAG41150.013.8999441
Produced by FastQC (version 0.11.5)