FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10911039
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT67600.019.6898144
TATACTG80400.019.5025485
TACACTG91000.019.3751015
CTATACT54950.017.374324
GTGTACG16250.016.3151591
CCTACAC57500.015.9986353
TCTATAC54800.015.8966743
GTCTAAG44950.015.3662021
ATACTGT105750.015.2230086
GTGTAGG66950.015.2146981
CTTACAC63400.015.0594173
GTATAGG38200.014.97654151
CTTAGAC46700.014.923133
GTATAAG53250.014.8053911
CCTATAC34900.014.7768933
TCTACAC68050.014.7472543
CTAGTAC29400.014.6967453
CTAGGAC50000.014.6351143
CTAGCAC55100.014.5453133
GTATAGA60500.014.53035451
Produced by FastQC (version 0.11.5)