FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10265896
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG74350.019.9042555
CTATACT49950.017.887984
CCTATAC35300.017.4018253
GTATAGG33300.017.3994481
CTACACT58000.017.3309084
TACACTG77950.017.1044185
TAGACTG53000.016.5953245
CCTACAC60750.016.4314773
CTTACAC58800.015.9079523
GTACTAG27700.015.876781
CTAGGAC50450.015.7735883
GTATAGA53300.015.5855241
ATACTGT93750.015.1897256
TACACAG108500.014.8618655
TCTATAC48400.014.85520653
GTGTAGG58550.014.7841521
GTCCTAG48900.014.5610581
TATACAG74600.014.3167255
TAGACAG88000.014.2784445
GTATAGC31900.014.2240851
Produced by FastQC (version 0.11.5)