FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10265896
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117600.1145540535380448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG82700.018.0618215
TATACTG73200.018.0220415
CTATACT47550.017.9085524
GTGTAGG63200.017.689761
CTACACT62200.017.6181554
GTACTAG25850.017.0293881
GTATAGG35400.016.5804021
CCTATAC31950.016.1666663
TATACAG77150.015.5612965
CTAGACT46450.015.4775674
CCTACAC60150.015.43393
GTATTAG44950.015.3895021
CTAGGAC47350.015.036263
GCTATAC30800.014.9572883
TAGACTG56100.014.93040855
GTATAGA51600.014.8957371
TCTATAC48500.014.679733
GTGTATA83000.014.4800271
TAGGACT55300.014.3890094
GTGCTAG50000.013.8351611
Produced by FastQC (version 0.11.5)