FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31750452
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG27700.035.62977243
TATGCCG31000.032.2880646
CGTATGC38300.025.95138444
CTCGTAT46050.021.35632742
CGCCAAT53700.020.14021933
TATCTCG55000.018.83634839
GTATGCC54300.018.43338845
GTATCGA26250.016.7994564
CGTATCG30750.015.8205273
CCCGTAT46650.014.7850871
CCGTATC40950.014.5294672
ACACGTC73550.014.03750513
ACGCCAA73800.013.94362632
TATCGAA34100.013.3426765
ATCGCGC33100.012.6898367
ATGCCGT88050.012.48066947
GTCCGAT24950.012.3487041
GATCGGA82400.012.109521
ACGTCTG86300.012.08516615
CGTGTTA35350.012.07938859
Produced by FastQC (version 0.11.5)