FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29402004
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37750.047.374648
TCGTATG39900.044.64632845
CGTATGC45750.039.4729446
GTATGCC58400.031.16248347
CTCGTAT56450.031.06120744
ACGTCCG66000.027.7888832
CGCACAT71500.024.5740637
ACACGTC76800.024.24364313
GTCACGT75950.024.24013329
CGTCCGC82050.022.3530333
TCCGCAC84250.021.68620535
GTCCGCA85800.021.53905534
GCCGTCT89250.020.70465751
ACGTCTG89600.020.62391915
CGTCTGA90100.020.43195516
TCACGTC95850.019.53620330
CACGTCT95500.019.53294214
TCTCGTA90600.019.46927843
ATGCCGT96550.018.92165449
CGTATCG27350.018.8110033
Produced by FastQC (version 0.11.5)