FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26513135
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33850.044.04426645
TATGCCG37900.039.70697848
CGTATGC43700.034.5971246
CGTGAAA45250.033.49334333
CTCGTAT44000.032.8499744
AACGATC44600.031.94023738
TGAAACG48600.031.11233535
GAAACGA49650.030.24276736
AAACGAT49800.029.80014437
GTATGCC56550.026.73552947
ACGATCT60200.023.66305239
CACGTGA72900.021.269931
ACACGTC72900.021.21964513
ACGTGAA74900.020.70217932
GTCACGT78300.019.57957329
ATGCCGT81850.019.07013749
GTATCGA20100.018.979524
TCACGTG81750.018.83876830
ACGTCTG82500.018.7079215
GCCGTCT86050.018.46481951
Produced by FastQC (version 0.11.5)