FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31387905
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35250.040.7068543
TATGCCG35550.040.36307546
CGTATGC43000.033.53282544
CGGCTAC54800.026.63433333
ACGGCTA57500.025.38387932
CTCGTAT56600.024.98155442
GTATGCC59250.024.6313245
ACACGTC80250.018.62217513
ATGCCGT85250.017.77597847
ACGTCTG90300.016.62699115
GCCGTCT92100.016.5678549
CGTCTGA89750.016.41721216
GATCGGA89000.016.364021
TCTCGTA92250.016.20034841
GCTACAT92300.016.00273935
ATCGGAA92250.015.858612
TCACGGC94650.015.71625730
CGTATCG25050.014.9495613
CACGTCT102600.014.73607814
GTATCGA24550.014.3987834
Produced by FastQC (version 0.11.5)