FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32034548
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26200.028.9863348
TCGTATG27600.027.38929245
CGTATGC33500.022.6699446
CGTATCG29350.017.1714173
TATCTCG47550.016.26690741
CTCGTAT46750.016.24487144
GTATCGA29350.015.8599334
GTATGCC48850.015.61807147
CCCGTAT43600.015.3371631
CCGTATC38100.014.9732842
GTCCGAT25050.012.2990931
TATCGAA41550.012.2138935
TCACGTG71300.011.53619530
ATCGCGC36650.011.26988367
CGTGTTA38100.011.20750559
ACACGTC73750.011.15203613
GTCACGT75200.010.98446929
CTCCGAT69050.010.7490561
TACATCG40450.010.64371564
TCCGATT57150.010.5945932
Produced by FastQC (version 0.11.5)