FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32132097
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26800.031.34177843
TATGCCG28750.030.67616746
CGTATGC40150.022.22786544
CTCGTAT44000.019.32900842
TAGCTTA55600.018.00418934
GTATGCC53250.017.2195645
ACTAGCT59000.016.43279532
TATCTCG53800.016.19911639
GCTTATC70350.013.78167636
GTCACTA73350.013.45645329
CACTAGC74000.013.196431
GTCCGAT30150.012.7733441
TCACTAG80550.012.64469730
TATCGCG5551.8189894E-1212.61279239
TCCGATT57350.012.3274112
ATCGCGT4953.43789E-1012.02033540
AGCTTAT97200.011.451135
ACACGTC88750.011.23896613
ACGTCTG90350.011.0010815
CCGATTG31250.010.9755923
Produced by FastQC (version 0.11.5)