FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27364268
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25850.037.90896243
TATGCCG28800.034.6330946
CGTATGC34900.028.57984544
CTCGTAT44450.021.73165742
GTATGCC50500.020.09769645
CACTTAG59250.017.13140331
ACTTAGG61800.016.76446532
ATGCCGT66750.015.93900447
TCACTTA65650.015.88780930
GTATCGA15700.013.8214354
GCCGTCT75400.013.507050549
CGTATCG20850.012.9252873
TCTCGTA81100.012.90373341
GTCCGAT19350.012.6657621
CGTCTGA83300.012.35231416
ACACGTC85850.012.31161513
CCGTAAT25050.011.8802471
TAGGCAT86500.011.85603835
ACGTCTG90200.011.48498315
CCCGTAT36150.011.2347641
Produced by FastQC (version 0.11.5)