FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25267135
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG15500.017.838413
CCCGTAT29450.016.6430321
GTATCGA18850.014.668164
GTCCGTT25400.014.1968891
CCGTATC26000.013.19242
TCCGATT38050.012.8778822
CTCCGAT47100.012.8592281
ATCGCGC18250.012.85065167
TATCGAA23700.012.7002085
TCGTATG14500.012.55099245
CCCCTAT32550.012.3690551
GTCCCGT38300.012.2488611
TATGCCG14150.012.11945848
CTCCGGT55300.011.83875751
GACGATA20700.011.835690556
GTCCGAA27350.011.7766071
CTCCAGT247700.011.6746461
CGATAGA15800.011.51847536
CTCCTGT215550.011.2882461
GCGATAG17500.011.1994835
Produced by FastQC (version 0.11.5)