FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32003267
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28350.034.9367243
TATGCCG30550.034.0238846
CGTATGC37200.027.753844
GTATGCC57150.018.67770645
CTCGTAT57350.017.33180842
ACACGTC86850.014.10406513
ACGTCTG92350.013.37775315
ATGCCGT81700.013.36506747
CGTCTGA92700.013.28955216
CCCGTAT41050.012.5372491
TCTCGTA85750.012.32650341
GTATCGA22300.012.2416854
ATCTACG26300.012.1116463
CGTATCG25500.012.0779323
GTCCGAT29250.011.9693961
AATCTCG92100.011.59079939
CCGTATC33050.011.4367992
TTGAATC105250.011.30670636
TCCGATT50700.011.18302252
CACGTCT113450.011.13639714
Produced by FastQC (version 0.11.5)