FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32800776
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39600.043.65839845
TATGCCG40900.041.8429448
CGTATGC46300.037.03830346
GTATGCC63350.027.29084447
CTCGTAT67550.025.6977844
ACACGTC91150.023.11463513
CGTCTGA99750.020.98154816
ATCGGAA109450.018.8984532
GATCGGA107250.018.8682691
ACGTCTG110750.018.80259715
AATCTCG99100.018.50612441
ATGCCGT96250.017.85328349
CACGTCT120150.017.62279914
TCTCGTA99650.017.4901443
CGTATCG25000.017.219283
CACACGT123800.017.04689212
GTATCGA26000.016.8263474
GCCGTCT104900.016.18095851
CCGTATC36950.015.1551452
CACATGT138950.014.91244331
Produced by FastQC (version 0.11.5)