FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31484414
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT460640.1463073125642421TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67450.053.23586345
TATGCCG70600.051.10830748
CGTATGC78600.046.21812446
CTCGTAT78200.043.7253844
ACCCGTC93750.038.41750732
GTATGCC94400.038.40831447
CGTCCCG101450.035.50147235
TCCCGAT97550.035.0189337
GTCCCGA110350.032.57470336
CACCCGT115900.031.40721931
ATGCCGT120650.030.37084649
CCCGATC110400.029.67470438
GCCGTCT124350.029.29834751
TCACCCG125300.029.19058230
ACACGTC127850.029.1542313
CCGATCT113150.028.92209639
TCTCGTA116450.028.58189243
CCGTCCC128250.028.5740434
CGTCTGA134950.027.54251716
ACGTCTG135500.027.35301615
Produced by FastQC (version 0.11.5)