FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31726993
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA356440.11234597618501067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43050.051.78530543
TATGCCG47050.047.7541246
CGTATGC50600.044.19649544
GTATGCC58300.038.65931345
CTCGTAT57650.037.51829542
ATGCCGT84250.027.41638247
ACACGTC95550.023.99129113
GCCGTCT95900.023.9763449
ACGTCTG97650.023.26027115
CGTCTGA99050.022.93186416
TTGTAAT104200.022.13634135
CTTGTAA108750.021.24222834
GTAATCT107550.020.50271837
CACGTCT112450.020.35447514
TGCCGTC118550.019.86776448
TAATCTC113000.019.57569738
CACACGT118850.019.49391412
GATCGGA117650.019.2843071
ACGACTT97600.018.2595021
GTTCGAC114050.018.1376829
Produced by FastQC (version 0.11.5)