FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31634417
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36500.043.05177348
TCGTATG38400.040.1926845
CGTATGC44400.035.7070146
TCCGTAT51550.030.28212237
CGTATCT54100.028.27183339
CCGTATC58150.026.4236138
CTCGTAT58500.026.08384344
GTTCCGT61950.026.0462535
TATCTCG60350.025.45935841
GTATGCC63650.024.90795147
AGTTCCG71850.022.9444534
TTCCGTA76700.020.90032236
ACACGTC87800.018.89430813
ATGCCGT89950.017.93651249
CGTCTGA95100.017.5175616
GCCGTCT97900.016.69451351
ACGTCTG99750.016.59558715
CACGTCT109300.015.4977614
TCTCGTA101650.015.32125343
GTATCTC104100.015.26411140
Produced by FastQC (version 0.11.5)