FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28159346
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG23350.021.2843363
GTATCGA23650.020.2743284
CCCGTAT37800.019.079251
TATCGAA29950.016.0096135
ATCGCGC28200.015.51602467
CCGTATC38400.015.4947312
GTCCGTT28600.014.4444771
CTCCGAT51050.014.0586621
TCCGATT42850.013.8039112
CGTGTTA31800.013.64825459
ATCTACG21050.013.63555563
GTCCGAT19750.013.4720091
ACCTACG22950.012.96249838
TACATCG36450.012.29209264
CATCGCG37600.012.10247766
ATGCGTA36550.012.06576557
TCGCGCC39250.011.95048268
ATAACGT24300.011.66711760
GTCCTAT42500.011.532541
CCGTAAT33200.011.4940821
Produced by FastQC (version 0.11.5)