FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25836176
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA23400.021.2386044
TATCGAA26100.020.5166725
CCCGTAT35100.019.9470621
CGTATCG26750.019.6255863
ATCGCGC28100.017.5645167
GTCCGAT21700.016.7775921
CCGTATC34700.016.7432142
TCCGATT45500.015.4612582
CGTGTTA31800.015.1891159
CATCGCG35950.014.60561366
CTCCGAT47250.014.4473711
TACATCG35600.013.86401564
ACATCGC40750.013.056965
GTCCGTT29000.013.0371241
TCGCGCC38750.012.55660468
CTCCCGT57750.012.063051
CCGATTG25000.012.0396423
CCGTAAT24200.012.0065641
CGATAAC23150.011.94406858
CTCCGGT55200.011.7958681
Produced by FastQC (version 0.11.5)