FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27218920
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34150.043.4530943
TATGCCG36550.040.5989246
CGTATGC40500.036.63987444
CTCGTAT52500.027.46569642
GTATGCC57750.025.93761845
ACGATCT64500.022.46601337
CACGATC73500.019.85793936
TCACGAT79550.018.9197135
ACACGTC84300.018.4332413
ATGCCGT87050.018.41334347
ATCACGA83400.018.33989334
ACGTCTG85850.017.93720215
CGTCTGA85300.017.88900616
CGTATCG24350.017.391553
TCTCGTA83650.017.32197841
GCCGTCT90750.017.0841749
GTATCGA22100.016.7867074
CATCACG96750.016.42424833
CACGTCT101300.015.546914
CCGTATC30000.015.5161182
Produced by FastQC (version 0.11.5)