FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31139239
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26650.030.07291246
TCGTATG27550.028.32875343
CGTATGC37000.022.51216944
ACCGATG48550.017.08648932
CTCGTAT46100.016.9300942
GTATGCC53400.015.66373345
CGTATCG24000.014.728763
TATCTCG54250.014.7097239
CCCGTAT40050.013.6374871
TCACCGA63950.013.51895530
GTATCGA24950.012.9055184
GATGTAT70850.012.696362535
CACCGAT67000.012.69461731
CGATGTA73300.012.1288434
ACACGTC74000.011.82382713
CCGTATC35950.011.4879712
ATGTATC78400.011.38434536
CCGATGT78100.011.02492633
CGTCTGA79550.010.91093516
ATGCCGT78350.010.89903547
Produced by FastQC (version 0.11.5)