FastQCFastQC Report
Fri 27 Jan 2023
EGAF00004980244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142179608
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGG17641801.2408108482054614No Hit
CGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGA15364411.080633869802201No Hit
GGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAG10877400.7650464193149273No Hit
CGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGG10600400.7455640192790516No Hit
GTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGG8568090.6026243932252225No Hit
GGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGAT7765800.546196470031061No Hit
CTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATAG7579690.5331066885484731No Hit
CACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATAGCT6994950.4919798344077584No Hit
GCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATA3995660.2810290488351888No Hit
GGGGGTTGTTGCTTTGAGGGAAGTTACGCTTATTTTTTTTTTGTCTTTTG1916170.13477108475358857No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC1906040.13405860564758343No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC1577400.11094417984328668No Hit
CAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATAGCTTGAGCCCAGGAGTT1576700.11089494634139097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)