FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29207772
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG116600.019.4177841
CTGCGGA105050.019.3514251
ACGAGGA105600.019.1311911
TCGGCGC142500.018.9799068
CATGGCG105850.018.9569131
CGGCGCA103150.018.9427229
GACTGCG111450.018.8815021
GCGCGAT99250.018.7877861
GATCGCG104800.018.7556821
TGCGTGG84950.018.7457071
ATGGGAG124550.018.7353481
GACTAAC112450.018.6388131
CGTGAGC91700.018.5464881
CCTTACG142250.018.4878981
ATAGACC92150.018.4787351
CCGGTAG71800.018.4458481
TGACAAC101050.018.3907091
TGGCCAG106500.018.3773381
CTCGAGG109150.018.3548851
TTGAACG85100.018.3544671
Produced by FastQC (version 0.11.5)